Evolutionary analysis indicates that DNA alkylation damage is a byproduct of cytosine DNA methyltransferase activity

DNMT 1 ( DNA ( cytosine - 5 - ) - methyltransferase 1 ) )

DNA ( cytosine - 5 ) - methyltransferase 1 - [ Isoform 1 ]

The murine equivalent of the human DNMT1 (DNA [cytosine-5]-methyltransferase 1; Swiss-Prot accession number: P26358) gene was deleted in ES cells via gene targeting [1]. Dnmt1-/cells possessed dramatically decreased genomic methylation and were viable; however, the mutation caused a homozygous lethal phenotype when introduced into the germline (see Table of experimental models for DNMT1) [1]. C...

Direct reversal of DNA alkylation damage.

3.5.1. Alkylation Chemotherapy 10 3.5.2. Inhibition of hAGT by O6-Benzylguanine 10 3.5.3. Developing More Potent Inhibitors 10 3.6. Application of hAGT in Biotechnology 10 3.6.1. Quantification of AGT 10 3.6.2. Radioactive Imaging of Tumors 11 3.6.3. hAGT Fusion Proteins in Biotechnology 11 4. AlkB Proteins 11 4.1. Oxidative Dealkylation by AlkB 11 4.1.1. E. Coli AlkB 11 4.1.2. Puzzle of AlkB 1...

Selectivity of Human DNA Maintenance Methyltransferase Endogenous Cytosine Damage Products Alter the Site

Alterations in cytosine methylation patterns are usually observed in human tumors. The consequences of altered cytosine methylation patterns include both inappropriate activation of transforming genes and silencing of tumor suppressor genes. Despite the biological effect of methylation changes, little is known about how such changes are caused. The heritability of cytosine methylation patterns ...

Endogenous cytosine damage products alter the site selectivity of human DNA maintenance methyltransferase DNMT1.

Alterations in cytosine methylation patterns are usually observed in human tumors. The consequences of altered cytosine methylation patterns include both inappropriate activation of transforming genes and silencing of tumor suppressor genes. Despite the biological effect of methylation changes, little is known about how such changes are caused. The heritability of cytosine methylation patterns ...